Chemical cross-linking coupled with mass spectrometry (CXMS) is widely used in protein interaction analysis, structure identification and kinetic studies. However, there are fewer types of cross-linking agents available for CXMS, which mainly target protein N-termini and lysine residues, resulting in limited structural information that this technology can provide.
Arginine is abundant in proteins (>5%) and plays an important role in protein interactions and structural analysis. The team of Professor Lei Xiaoguang of Peking University developed a selective cross-linking agent targeting arginine residues: ArGOs to help obtain richer protein structure analysis information.
ArGOs cross-linking agent selectively reacts with arginine residues on the protein surface (Figure 1), filling the gap that most cross-linking agents in CXMS technology are lysine-based, and has the following characteristics:
High reactivity and stable cross-linked products;
More number of cross-linked peptide identifications can be obtained, providing rich information for protein structure analysis.
Structures of ArGO1-3 compounds and of two major cross-linking products[1]
J&K Scientific exclusively sells the new cross-linking agent, the scientific research result of Professor Lei Xiaoguang.
Professor Lei Xiaoguang currently works at the School of Chemistry, Peking University. His research fields include chemical biology, natural product synthesis, synthetic biology and innovative drug research and development. He has hosted/participated in a number of important national scientific research projects. One of the main research directions of his research group is to develop a new generation of efficient biocompatible reactions and use chemical cross-linking combined with mass spectrometry technology to systematically study complex protein structures and protein interactions. Research results have been published in academic journals such as Nature and Cell.
References
向阳 翟
