Peroxynitrite anion (ONOO-) plays a key regulatory role in many physiological processes and is closely related to the occurrence of oxidative damage, ischemic stroke, neurodegenerative diseases, cancer and other diseases.

In order to explore the biological mechanism of ONOO- in disease occurrence, it is particularly critical to find a method that can monitor endogenous ONOO- content in real time.

The scientific research team of Associate Professor Li Xin of Zhejiang University has developed a two-photon fluorescent probe for detecting ONOO- content in living cells and vivo - NP3 (λex 375nm; λem 470nm), which leads to a dearomatization reaction by oxidizing p-hydroxyaniline structures. Generates strong fluorescence to detect ONOO- content (Figure 1); at the same time, NP3 has excellent biocompatibility and can penetrate the blood-brain barrier to achieve real-time detection of nitrative stress response during ischemic/hemorrhagic cerebrovascular injury in living mice. monitoring[1][1]

Product advantages
High sensitivity: detection limit as low as 5nM;
Fast response: reaction can reach equilibrium within seconds;
Strong specificity: other oxidizing species have no obvious response to the probe;
Within the pH range of 6-8, the fluorescent product is less affected by pH.

J&K Scientific exclusively sells the two-photon fluorescent probe NP3 developed by Professor Li Xin:

Professor Li Xin once worked in the laboratory of Professor E.J. Corey (postdoctoral fellow) and is now engaged in medicinal chemistry research at Zhejiang University. In response to the problem that most biomolecules are difficult to detect in situ in vivo, her laboratory designs fluorescent probes based on the chemical reactivity of biomolecules under the guidance of the drug-like principle, providing sensitive and reliable detection tools for in-depth study of the molecular mechanisms of related pathological processes. and technology.