The TPQL-1 and TPQL-2 fluorescent dyes developed by the Zhang Xiaobing-Yuan Lin research group of Hunan University were developed on the 6-ethyl-2-dimethylaminonaphthalene (acedan) structure through a single atom substitution and electron acceptor combination strategy. of new two-photon dyes (Figure 1). [1][1]

Two-photon fluorescent dye imaging has been widely used in recent years due to its deeper tissue penetration (up to 1,000 μm) and smaller tissue absorption. It can also well suppress photodamage, photobleaching and spontaneous background fluorescence during sample imaging. Imaging and detection of biomolecules in biological systems. [2]

Compared with acedan and other traditional two-photon fluorescent dyes, TPQLs series two-photon dyes have:

High fluorescence quantum yield and good solvent insensitivity: reduce false signals caused by environmental changes in imaging and improve imaging signal-to-background ratio.
For accurate detection of biological analytes in complex bioimaging environments.
These dyes can be well used for the development of two-photon fluorescent probes and the detection of biological analytes, such as: hydrogen sulfide probe (TPQL-N3) and aminopeptidase probe (TPQL) developed based on TPQL-1 azide substitution. -APN) for dynamic monitoring of hydrogen sulfide and aminopeptidase during liver development and growth (Figure 2).

Figure 2 TPQL probes for accurate liver-development imaging (A and C) Two-photon images of H2S (A) and APN (C) in 2, 4, 6, 8 and 10-dpf zebrafish: the zebrafish were cultivated with TPQL-N3 (10 mM) and TPQL-APN (10 mM) for 1 h at 25C. lex = 810 nm, lem = 500–550 nm. Scale bar, 100 mm. (B and D) Inhibitor experiment of H2S (B) and APN (D). (E–G) Normalized fluorescence intensity of the fluorescence images (A)–(D). Error bar = RSD (n = 5). (H) Co-location experiment: images of 6-dpf transgenic zebrafish preincubated with TPQL-APN for 60 min. (H1) green channel for TPQL-APN, lex = 405 nm, lem = 500–550 nm. Scale bar, 100 mm. (H2) red channel for transgenic red fluorescent protein, lex = 560 nm, lem = 570–620 nm. (H3) overlay channel. (H4) local amplification of (H3). Scale bar, 20 mm. (I and J) Western blot analysis showing CBS (I) and APN (J) expression in 2, 4, 6, 8, and 10-dpf zebrafish. The CBS and APN relative abundance was normalized with actin-relative abundance. ^[1]

Professor Yuan Lin is a professor and doctoral supervisor at the School of Chemistry and Chemical Engineering, Hunan University and the State Key Laboratory of Chemical Biosensing and Metrology.Currently, he has published more than 100 papers in international academic journals, with a total citation of more than 12,000 times, and an h-index of 58.Among them, the first or corresponding author from 2012 to presentJ. Am. Chem. Soc.（10 articles）、 Nat. Commun.（1 articles）、 Angew. Chem. Int. Ed.（10 articles）、CCS Chem.（1 articles）、Chem. Sci.（4 articles）、Anal. Chem.（10 articles）、Adv. Funct. Mater、BiomaterialsPublished more than 70 papers in journals and other journals. Mainly engaged in research on small molecule fluorescent probes and their applications.

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