Human serum albumin (HSA) is a protein in human plasma. HSA concentration in the blood or urinary system is a reliable indicator of various diseases such as kidney disease, hypoalbuminemia, etc.
The presence of excess HSA in the urine can lead to microalbuminuria, an early marker of cardiovascular disease and kidney disease. On the other hand, low levels of HSA in plasma can lead to hypoalbuminemia, a symptom of cirrhosis and chronic hepatitis.
Therefore, the selective detection of HSA is a need for biochemical research and clinical research, and is of great significance in proteomics research, diagnosis, therapy, etc.
Professor Zhang Pingyu of Shenzhen University developed a metal iridium complex-HSA-Ir fluorescent probe (Figure 1), which has obvious fluorescence response to different concentrations of HSA [1][1][1].The gel of different concentrations of HSA treated with Coomassie blue G250 (100 mg L1 , 1 h) or Ir1 (5 mM, 2 min) after separation. (b) Selective staining: the gel of HSA, blood serum and mixture of other proteins (hemoglobin, transferrin, myoglobin, g-globulin, hemin, lysozyme, apo-transferrin, methemoglobin and holo-transferrin) was stained with Ir1. Lanes A and D: mixture of other proteins; lane B: HSA; lane C: HSA + mixture of other proteins; lane E: blood serum; lane F: blood serum + mixture with other proteins. (c) The gel of HSA (10 mg) per lane and then treated with Ir1 for different incubation times after separation. Twice in parallel with each experiment. (d) The gel of different concentrations of HSA and then treated with Ir1 after separation.
Zhang Pingyu, Distinguished Professor of Shenzhen University, Youth Yangtze River Scholar of the Ministry of Education, Royal Newton Scholar, and doctoral supervisor. He has long been focusing on the basic research of metal complexes in the field of tumor treatment, and has carried out systematic research on the photodynamic therapy, sonodynamic therapy and mechanisms of metal complexes, providing new structures and new strategies for the development of efficient metal drugs. At the same time, metal drugs are used as tools to reveal the occurrence and development of tumor diseases, and chemical biology methods and technologies are combined to elucidate the mechanism of metal drugs intervening in cancer stem cell pathways, providing a theoretical basis for clinical research on metal drugs.
Product list
10 MG
- Yi Wang, Huaiyi Huang, Ge Chen, Haijie Chen, Tingting Xu, Qian Tang, Hailiang Zhu, Qianling Zhang and Pingyu Zhang, Chem. Commun., 2018, 54, 3282—3285.
向阳 翟
