EC series near-infrared fluorescent dyes are a new type of high-performance fluorescent dyes developed by the team of Professor Yang Youjun of East China University of Science and Technology (Figure 1) [1-7] to meet the application needs of infrared imaging, photothermal ablation and other applications in the biomedical field. Using self-built fluorescence microscopy equipment, fluorescence imaging of cells or living bodies can be performed.
figure 1 EC dyes[1]
Compared with traditional cyanine near-infrared fluorescent dyes (such as Cy5, indocyanine green), EC series near-infrared fluorescent dyes have obvious advantages in key indicators such as absorption and emission wavelength, fluorescence intensity, photostability, and environmental sensitivity:
Narrow peaks and high fluorescence intensity: The peaks of the absorption and emission spectrum are narrow (FWHM=45 nm), the fluorescence intensity is high (ε×φ>40,000 M-1cm-1), and the fluorescent labeling and imaging effects are good;
Good photostability: the photobleaching ratio of EC fluorescent dyes is only 1/10-1/1000 of that of indocyanine green in the same time;
Biocompatible: EC fluorescent dye with λex <800 nm has good cell membrane permeability and is convenient for staining cells or biological tissues.
It is classified as follows according to usage:
Suitable for fluorescence microscopy imaging at the cellular level
It has excellent luminescent properties, resistance to photobleaching, antioxidant, and resistance to biothiol addition.
It has the ability to cross cell membranes and is located in cell mitochondria.
| EC fluorescent dye | Fluorescence | Molar Extinction Coefficient |
|---|---|---|
| EC640, 97%, Near-infrared fluorescent dye, liposoluble | λex 640 nm; λem 659 nm | >130,000 cm -1M-1 |
| EC710, 97%, Near-infrared fluorescent dye, water-soluble | λex 712 nm; λem 740 nm | > 59,100 cm -1M-1 |
Suitable for in vivo fluorescence microscopy imaging of cells and small animals
It has excellent luminescent properties, resistance to photobleaching, antioxidant, and resistance to biothiol addition.
Cannot cross cell membranes.
It also has photothermal, photoacoustic and photodynamic properties.
| EC fluorescent dye | Fluorescence | Molar Extinction Coefficient |
|---|---|---|
| EC825, 97%, Near-infrared fluorescent dye, liposoluble | λex 825 nm; λem 857 nm | >269,000 cm -1M-1 |
| EC835, 97%, Near-infrared fluorescent dye, liposoluble | λex 835 nm; λem 870 nm | >226,000 cm -1M-1 |
| ESi860, 97%, Near-infrared fluorescent dye, water-soluble | λex 862 nm; λem 895 nm | >137,960 cm -1M-1 |
| EC870, 97%, Near-infrared fluorescent dye, water-soluble | λex 835 nm; λem 923 nm | >185,200 cm -1M-1 |
| ESi880, Near-infrared fluorescent dye, water-soluble | λex 877 nm; λem 916 nm | >180,520 cm -1M-1 |
Suitable for in vivo fluorescence microscopy imaging of small animals
It has excellent luminescent properties, resistance to photobleaching, antioxidant, and resistance to biothiol addition. Cannot cross cell membranes. It also has photothermal and photoacoustic properties.
Among them, EC900 fluorescent dye has weak photodynamic properties, Ka<0, and is suitable for extremely acidic systems.
EC1200 fluorescent dye has no photodynamic properties.
| EC fluorescent dye | Fluorescence | Molar Extinction Coefficient |
|---|---|---|
| EC900, 97%, Near-infrared fluorescent dye, water-soluble | λex 901 nm; λem 976 nm | >163,000 cm -1M-1 |
| EC1200, 97%, Near-infrared fluorescent dye, liposoluble | λex 1208 nm; λem 1280 nm | >391,000 cm -1M-1 |
Professor Yang Youjun is a professor and doctoral supervisor at the School of Pharmacy at East China University of Science and Technology. His research group focuses on the design and development of fluorescent molecular probes, surgical navigation dyes, etc. focusing on major diseases related to biological redox. Specific work covers medicinal chemistry, pharmaceuticals, medicinal chemistry and biology, etc. The results were published in Nat. Comm.、JACS（8 articles）、Angew. Chem. Int. Ed.（3 articles）、PNAS、Free Radic. Biol. Med.、Anal. Chem.、Org. Lett.、Chem. Comm.、J. Org. Chem.、Sens. Actuactors B Chem. and other high-impact journals in this field.
向阳 翟
