G-quadruplex (G4) fluorescent probes provide ideas for broadening viromics research on functional nucleic acids and developing new HCV infection detection. However, the traditional G4 dye has poor ability to cross cell membranes and is difficult to be directly used for live cell imaging.
Hunan University's Nie Zhou team developed a new type of living cell nucleic acid G-quadruplex (G4) near-infrared fluorescent probe - NIR-2 (λem 689 nm), which is based on the GFP (green fluorescent protein) chromophore. Above, a fluorescent chromophore with a D-A-A structure is derived. The molecule itself has a weak fluorescence signal. After combining with G4, it can generate igMFPs, so that the fluorescence is lit.
- High fluorescence quantum yield;
- Good transmembrane ability;
- Low cytotoxicity makes it ideal for live cell imaging.
Professor Nie Zhou is currently the Vice Dean and Director of the Degree Office of the Graduate School of Hunan University, and the Director of the Hunan Provincial Key Laboratory of Biomacromolecule Chemical Biology. He has long been engaged in basic research on life analytical chemistry, closely focusing on the needs of key mechanism research in biomedicine. Continue to carry out basic research on the creation of biomolecular tools and their application in life analysis. In recent years, he has published more than 100 academic papers as the corresponding author in high-level journals such as J. Am. Chem. Soc., Angew. Chem. Int. Ed., Science Advances, Nano Lett., etc., published 2 chapters in his personal monograph, and has been authorized by the National Invention He holds 7 patents and presides over projects such as the National Natural Science Foundation for Distinguished Young Scholars, Key Projects, and Excellent Youth Projects, as well as National Key R&D Plan Projects.
In addition, the probe successfully achieved high-contrast HCV RNA imaging in living mice, providing ideas for the application of fluorescent protein mimics in whole-animal imaging (Figure 1).
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References
- J. Am. Chem. Soc. 2021, 143, 46, 19317–19329.
向阳 翟
