High-fidelity in vivo fluorescence monitoring of physiological processes is crucial in disease diagnosis and biological research, but it faces extreme challenges due to aggregation-induced quenching (ACQ) and short-wavelength fluorescence. The development of high-performance, long-wavelength aggregation-induced emission (AIE) fluorophores has placed higher demands on precise optical bioimaging.

Chromophore-quinoline-malononitrile (quinolinenitrile, QM) is a new type of AIE structural unit that has emerged in recent years. It has infrared to near-infrared (NIR) emission, high brightness, good photostability and biophase remarkable features such as capacitance. They are highly decorable and easy to functionalize, and can be used as ideal dye masters for the development of fluorescent labels and fluorescent probes.

Quinolonitrile (QM) dye was independently developed by Guo Zhiqian-Zhu Weihong's research group at East China University of Science and Technology and has independent intellectual property rights. Relevant research results have won many awards such as the second prize of the National Natural Science Award and the first prize of the Shanghai Natural Science Award.

After nearly ten years of research and development, this type of products has been continuously enriched, and now more than 30 types have been developed. J&K scientific exclusively sells quinoline nitrile (QM) products developed by Professor Guo Zhiqian-Zhu Weihong:

Product information

Product name:
QM-FN-SO3, NIR Amyloid-ß Plaque Imaging Dye, 95%
QM-FN-SO3, Amyloid-β near-infrared imaging reagent
Item number: 9230967
Product Description:
Absorption wavelength: 500 nm; Emission wavelength: 720 nm
Application:
Near-infrared high-fidelity in situ imaging of Aβ plaques (Figure 1);

Figure 1 Near-infrared high-fidelity in situ imaging of Aβ plaques by QM-FN-SO3 (b, c) wild-type mice and (d, e) APP/PS1 mice 20 minutes after intravenous injection of QM-FN-SO3 Comparison of images. [1]

Alzheimer's disease is a severe degenerative disease of the central nervous system. Research has shown that the formation and deposition of beta-amyloid (Aβ) plaques in the brain play an extremely important role in the neuropathology of AD and are considered important pathological markers for early diagnosis and treatment of the disease. Currently known commercially available gold-labeled dyes thioflavin ThT/ThS are limited by (1) short emission wavelength (mainly in the 400-600 nm range); (2) fluorescence signal distortion caused by aggregation quenching; (3) binding proteins The former “always-on” background noise interference; (4) Inherent defects such as the inability to penetrate the blood-brain barrier (BBB).

Compared with the aforementioned dyes, QM-FN-SO3 has:

Combining background noise minimization and fidelity signal amplification to achieve ultra-high signal-to-noise ratio (S/N);
Significant binding ability to Aβ plaques and BBB penetration performance;
Near-infrared activatable AIE emission wavelength and excellent photostability.

Product name:
QM-β-Gal, 95%
Item number: 9233837
Application:
β-Galactosidase activity detection reagent. The activity of β-galactosidase is significantly increased in senescent cells and primary ovarian cancer cells, and can be used as an important biomarker.
QM-β-Gal has:

• Strong intracellular retention ability (12 h, Figure 2), can monitor endogenous β-gal in cells in situ, and perform long-term fluorescent labeling and tracing of ovarian cancer cells;
• Excellent water solubility;
• Fast response time (35 minutes);
• Low cytotoxicity and good permeability.

Figure 2 Suitability of QM-β-Gal for high-resolution long-term monitoring of β-gal overexpressing SKOV-3 cells. [2]

Product name:
QM-SO3
Water-soluble quinoline nitrile sulfonate precursor
CAS: 1800102-18-4
Item number: 9230961
Product Description:
Absorption wavelength: 420 nm Emission wavelength: 520 nm
Authorized patent: ZL201410512468.9
Appearance: orange-yellow solid

Product name:
QM, 95%
Quinolonitrile parent
CAS: 1402299-58-4
Item number: 9230960
Product Description:
Absorption wavelength: 420 nm Emission wavelength: 520 nm
Authorized patent: ZL201210199278.7; ZL201410512468.9
Appearance: orange-yellow solid

Professor, doctoral supervisor; winner of the National Outstanding Youth Fund, Young Yangtze River Scholar.
Mainly engaged in related research in the field of fine organic chemicals, committed to breaking through key technical bottlenecks in the innovative applications of dyes in cross-cutting fields such as biomedicine, information materials, and environmental ecology, focusing on the rational design and functional enhancement of near-infrared dyes, and developing high-performance, refined , cutting-edge basic research on near-infrared dyes that can be commercialized. So far, he has published more than 100 papers as the first or corresponding author in JACS, Angew, Nat. Comm. Nat. Protoc., with more than 8,000 citations, and an H-index of 46. He focuses on industrial application and transformation, and has applied for 18 invention patents. Authorized 10 items to replace commercially available traditional probe reagents, commercialize high-performance quinolonitrile dyes and fluorescent microspheres; develop high-end fluorescent diagnostic reagents to detect Alzheimer's disease Aβ protein plaques. He won the second prize of the National Natural Science Award in 2019 (2/5); the Shanghai Youth Science and Technology Outstanding Contribution Award, etc.

References:

1. W. Fu, C. Yan, Z. Guo, J . Z hang, H . Z hang, H . T ian, W. H. Zhu, J. Am. Chem. Soc. 2019, 141, 3171 – 3177.
2. K. Gu, W. Qiu, Z. Guo, C . Y an, S. Zhu, D. Yao, P. Shi, H. Tian, W. H. Zhu, Chem. Sci. 2019, 10, 3 98 – 4 05.