FM series fluorescent dyes include FM 1-43, FM 2-10, FM 5-95, and FM 4-64. They are a type of lipophilic styrene fluorescent dyes that are non-toxic to cells and are mainly used for living cell membranes, especially plant samples. Specific labeling of cell membranes, tracer imaging of neuronal cells, and tracking of vesicle-related biological processes such as endocytosis and exocytosis [1-13].
The FM series of fluorescent dyes have good water solubility and do not emit light in aqueous solutions. However, when combined with cell membranes and similar membrane structures, they emit bright fluorescence and are widely used for dye labeling of living cell membrane structures, especially plant samples. Compared with other cell membrane dyes, it has the advantages of high specificity, uniform staining and bright signal.
The FM series of fluorescent dyes can also track and label neuronal cells through fluorescent signals. In neurons that actively release neurotransmitters, the dyes will be internalized in recyclable protruding vesicles, so that the ends of neuronal cells are labeled, which can be used to study nerves. related physiological processes.
Taking FM 1-43 as an example, FM 1-43 fluorescent dye can be used for highly specific imaging of membrane structures in plant cells, including root tip cells of onion (Figure 1), tobacco (Figure 2), rice, Arabidopsis and other plants. High-definition imaging of cell membranes of various cells such as , leaf epidermal cells, etc.
Figure 1 Laser confocal imaging of onion epidermal cells stained with FM 1-43
Figure 2 Laser confocal imaging of tobacco leaf epidermal cells stained with FM 1-43
Reference:
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Dr. Qian Zhaosheng, professor, "Double Dragon Scholar" Distinguished Professor, doctoral supervisor, winner of the Zhejiang Provincial Outstanding Youth Fund, and senior member of the Chinese Chemical Society. Currently, he is mainly engaged in the design, synthesis and precise imaging applications of new molecular fluorescent probes targeting cellular substructures, as well as research on super-resolution imaging applications based on molecular fluorescence switch systems. He has presided over and completed a number of Zhejiang Provincial Natural Science Funds, including the National Natural Science Foundation Youth Fund, general projects, and the Zhejiang Provincial Distinguished Youth Fund. He has participated in two National Natural Science Foundation general projects and several Zhejiang Provincial Natural Science Funds, and has presided over and completed 1 project. Horizontal project; has published more than 110 papers in international academic journals such as "Journal of American Chemical Society" and "Chemical Science", including 2 highly cited papers and 5 articles selected as cover articles, h -index 40, the highest number of citations for a single article is 395, and the total number of citations is nearly 5,300. Won the third prize of the Zhejiang Provincial Natural Science Award in 2017, the "Publons Peer Review Award" in the chemistry discipline in 2018, and was selected into the 2021 ranking of the world's top 100,000 scientists and the world's top 100,000 scholars in academic influence in 2022 and 2023. Ranking list.