The crimp-sealed vial is a type of sample bottle used in instruments such as gas chromatography and liquid chromatography. Its sealing is achieved through the crimp cap. Here are the methods for crimp-sealed vial sealing:

1. Manual Crimper: The manual crimper is a common sealing tool used to manually crimp the crimp cap tightly onto the vial. This is a convenient sealing method suitable for small-scale sample processing.

2. Automatic Crimper: For large-scale sample processing, an automatic crimper is often used. The automatic crimper can efficiently and accurately crimp the crimp cap onto the vial, improving experimental efficiency.

3. Pressure Balance: During the headspace extraction and sealing process, it is important to maintain pressure balance inside and outside the sample vial to avoid excessive gas pressure differences during sealing.

4. Proper Septa Selection: The choice of septa is crucial when sealing crimp-sealed vials. Common septa materials include polytetrafluoroethylene (PTFE) and silicone. Choosing the appropriate septa helps ensure reliable sealing.

5. Check Sealing Effect: After sealing, it is necessary to check the sealing effect, ensuring that the crimp cap is evenly and tightly crimped onto the vial mouth to prevent gas leakage or external contamination.

6. Preventing Cross-Contamination: During the sealing process, attention should be paid to preventing cross-contamination to ensure accurate and reliable analysis results between different samples.

The choice of these methods depends on the specific requirements, scale, and equipment used in the experiment. During the sealing operation, careful, accurate, and standardized procedures are key to ensuring the success of the experiment.